A new fluorophor for tubulin which has permitted the monitoring of microtubule assembly in vitro is reported. DAPI (4',6-diamidino-2-phenylindole), a fluorophor already known as a DNA intercalator, was shown to bind specifically to a unique tubulin site as a dimer (KD(app) = 43 +/- 5 microM at 37 degrees C) or to tubulin associated in microtubules (KD(app) = 6 +/- 2 microM at 37 degrees C) with the same maximum enhancement in fluorescence. When tubulin polymerization was induced with GTP, the change in DAPI affinity for tubulin resulted in an enhancement of DAPI binding and, consequently, of fluorescence intensity. DAPI, whose binding site is different from that of colchicine, vinblastine, or taxol, did not interfere greatly with microtubule polymerization. It induced a slight diminution of the critical concentration for tubulin assembly due to a decrease in the depolymerizing rate constant. Moreover, DAPI did not interfere with GTP hydrolysis correlated with tubulin polymerization, but it decreased the GTPase activity at the steady state of tubulin assembly. Even at substoichiometric levels DAPI can be used to follow the kinetics of microtubule assembly innit.
(
mofaha ┐( ˘_˘)┌ ʅ(́◡◝)ʃ,
Thu 28 Jan 2010, 14:10,
archived)
Praise befor the gift of copy 'n' paste.
(
Sea Tramp the thinking woman's 8 Ace,
Thu 28 Jan 2010, 14:11,
archived)
What does that mean?I was just chatting.
( mofaha ┐( ˘_˘)┌ ʅ(́◡◝)ʃ,
Thu 28 Jan 2010, 14:13,
archived)
chatting me up more likethat kind of sweet talk always gets me aroused
(
pzyko Query failed.,
Thu 28 Jan 2010, 14:14,
archived)
bearing in mind that you have a little cartoon Hitler face next to your assumed name. Sea Tramp the thinking woman's 8 Ace,
Thu 28 Jan 2010, 14:41,
archived)
Me, too. I was just idly thinking:I met a traveller from an antique land
Who said: "Two vast and trunkless legs of stone
Stand in the desert. Near them on the sand,
Half sunk, a shattered visage lies, whose frown
And wrinkled lip and sneer of cold command
Tell that its sculptor well those passions read
Which yet survive, stamped on these lifeless things,
The hand that mocked them and the heart that fed.
And on the pedestal these words appear:
`My name is Ozymandias, King of Kings:
Look on my works, ye mighty, and despair!'
Nothing beside remains. Round the decay
Of that colossal wreck, boundless and bare,
The lone and level sands stretch far away".
(Who said: "Two vast and trunkless legs of stone
Stand in the desert. Near them on the sand,
Half sunk, a shattered visage lies, whose frown
And wrinkled lip and sneer of cold command
Tell that its sculptor well those passions read
Which yet survive, stamped on these lifeless things,
The hand that mocked them and the heart that fed.
And on the pedestal these words appear:
`My name is Ozymandias, King of Kings:
Look on my works, ye mighty, and despair!'
Nothing beside remains. Round the decay
Of that colossal wreck, boundless and bare,
The lone and level sands stretch far away".
Sea Tramp the thinking woman's 8 Ace,
Thu 28 Jan 2010, 14:20,
archived)
thanks wikithanwiki
It doesn't work does it
(It doesn't work does it
epiphany coming live from sweden,
Thu 28 Jan 2010, 14:11,
archived)
I knew what this waswithout having to look it up. It is a sad day for me.
edit 1: now I get why you said that. Apparently looking at the pic for more than 0.2 seconds was too much for me at first.
edit 2: but there ain't no whales so we tell tall tales and sing a whaling tune.
(edit 1: now I get why you said that. Apparently looking at the pic for more than 0.2 seconds was too much for me at first.
edit 2: but there ain't no whales so we tell tall tales and sing a whaling tune.
abandonnship,
Thu 28 Jan 2010, 14:38,
archived)